Evaluación de extractos acuosos de alóe vera y canela sobre el crecimiento micelial de moniliophthora roreri a nivel in vitro

Abstract

The present work consisted in the evaluation of the combination of aqueous extracts of aloe vera and cinnamon on the mycelial growth of moniliophthora roreri at the in vitro level, of the cocoa plantation that is part of the clonal garden of the UTMACH in the Santa Ines farm belonging to to the Faculty of Agricultural Sciences; in order to determine the best aqueous extract that inhibits the mycelial growth of the fungus M. roreri at the in vitro level, helping to improve the efficiency of the extract for the control of the disease that causes damage to the productivity of cocoa plantations in the province. del Oro, in turn obtain statistical data of reference to the results obtained in the Phytopathology laboratory. Data collection was carried out between the months of September and October 2021, carrying out 3 treatments T1 (control), T2 (PDA + ALOE VERA), T3 (PDA + CINNAMON + ALOE VERA), where each treatment three dosages were carried out in volume of solution to 10 ml, 20 ml, 30 ml, with 4 repetitions for each treatment evaluating the mycelial growth of the fungus M.roreri, where 1500 ml of PDA culture medium (potato-destroza-agar) was prepared, from which we use 820 ml for our experiment, we store the remainder in glass bottles in the refrigerator, for the preparation of the culture medium, 20 g of PDA is weighed for every 500 ml of purified water, it is placed in the microwave for 3 minutes until a small boiling and a translucent appearance, 60mg chloramphenicol is placed, stirred and calibrated with hot water up to 500ml in a beaker, finally the PDA is distributed with a test tube in glass bottles with an aluminum foil lid and sealed with tape to Around, the measurements used are 70-80-90 ml of culture medium, it is taken to the sterilizer (autoclave) where it will be purified and ready to inoculate the fungus and its different extracts. The data obtained were organized in a general table where a statistical analysis was carried out using the INFOSTAT program, resulting in a PARAMETRIC DCA-ANOVA, obtaining an Analysis of Variance where a significance of <0.001 was observed between the treatments carried out, affirming the alternative hypothesis (< 0.05) that at least of the treatments is different, instead in the Test of comparison of means Test: Tukey the treatments T3 (cinnamon + PDA) 30 ml AND T9 (PDA + CINNAMON + ALOE VERA) that established differences to the other treatments On the other hand, the LEVENE test shows significance values of <0.001 where it is mentioned that some of the treatments are different and that they do not meet the assumptions of normality. The information obtained through the collection of laboratory data gives way to the use of organic methods for the control of said disease (M. roreri), in turn in the implementation of a new methodology for future research work linked to the search for new strategies. management and conservation of the environment in its genetic diversity