Prototipo de sistema de inmersión temporal en fase de multiplicación en la micropropagación de banano clon williams

Abstract

Bananas are a key for Ecuador's agricultural production. It's common to require large quantities of plants that are obtained by somatic organogenesis. The use of different TIS has been reported to overcome limitations of in vitro cultures in permanent contact with the growing medium. The purpose of this work was to evaluate the multiplication of banana (Musa AAA cv. Williams), by using a TIS prototype (TISP) of local origin compared to TIS RITA® and a semisolid system (SS). At 21 days, data were recorded on the multiplication rate by type of shoot and total shoots, length of pseudostem and leaf 1, total roots per explant, fresh mass, ash, total soluble phenolic compounds (TSPC) and antioxidant activity (AA). The vitroplants of the PSIT presented a greener, more vigorous and uniform appearance. No significant differences were found with respect to total multiplication rate between TIS. The vitroplants obtained in TISP presented the longest pseudostem and leaf 1 length, also the highest ash content. No significant differences were found between TIS regarding to fresh mass. The highest TSPC and the highest AA were evidenced in shoots obtained in TISP. The use of PSIT allows the obtaining of vitroplants of superior quality to the standard, at the same time that the optimization of the multiplication phase of axillary shoots of Musa cv. Williams.