Importancia de la técnica pcr para el diagnóstico de enfermedades del camarón <i>litopenaeus vannamei</i>

Abstract

The study of different origins of the main diseases that affect shrimp farms. The effect that these can cause on crops and one of the techniques that makes possible a reliable diagnosis of these pathologies. Pathologies of parasitic origin are usually less complicated to diagnose since they can be seen with techniques such as microscopy, such is the case of gregarine infection that affects many species of penaeids and other crustaceans, these parasites are visible by microscopy techniques in analysis of intestinal content and rectal ampulla. Bacterial diseases and viral diseases are the ones that require the greatest use of this technique for their diagnosis. The study showed that the use of PCR is necessary for the detection of viral diseases such as White Spot "WSSV", Infectious Hypodermal and Hematopoietic Necrosis "IHHNV", Taura Syndrome "TSV", Hepatopancreatic Parvovirus (HPV). As well as diseases of bacterial origin, mainly of the Vibrio genus. It was possible to determine that the PCR molecular technique is of great importance for the precise detection of the aforementioned pathogens, it demonstrated 99.99% certainty when it comes to yielding results in the identification of viruses and bacteria such as WSSV and Vibriosis.