Interpretación de la técnica pcr-tr para la determinación de su reproducibilidad, especificidad y sensibilidad con el histoplasma capsulatum.

Abstract

Birds of the order Columbiforme (pigeons) are vectors of Histoplasma capsulatum, a fungus of clinical study, causing the disease Histoplasmosis, imported from endemic areas of Central and South America, Africa and Asia, although at present this disease is spread over all continents, due to migration and tourism. The aim of this research was to interpret the PCR-TR technique through bibliographic analysis to determine the reproducibility, specificity and sensitivity to Histoplasma capsulatum with the amplification of ribosomal DNA To fulfill this objective, both methods and techniques from primary and secondary bibliographic sources of the PCR-TR process, and from the programs for the elaboration of initiators and probes Lightcycler Probe Design Software, Fluorescence Resonance Energy Transfer, the Dneasy Plant Mini Kit method, among others As a result, it was possible to evaluate the reproducibility, specificity and sensibility of the technique in the Histoplasma capsulatum with regression curves, melting, analysis of immunodiffusion and serology. Concludes that the PCR-TR technique is a great utility in the determination of the Histoplasma capsulatum and a useful tool that strengthens the diagnosis.