Identificación de escherichia coli enterohemorragica 0157:h7 en adulto mayor mediante técnicas microbiológicas y moleculares para diferenciación de otros grupos patógenos.

Abstract

Escherichia Coli is a bacterium with chronic pathogenicity in the human organism1; One of the most lethal is the enterohemorrhagic Escherichia coli (EHEC) serotype O157: H7, which causes from simple diarrhea to hemorrhagic colitis, which could lead to hemolytic uremic syndrome (HUS), known to cause kidney failure in children and adults. Objective: To identify the strain Escherichia coli Enterohemorrhagic 0157: H7 in older adults, using microbiological and molecular techniques for its subsequent differentiation from other pathogenic groups. Methods: the descriptive method was used for a clinical case that required the collection of bibliographic information. The qualitative and quantitative microbiological methods include stool culture, isolation, detection of the causative agent and susceptibility testing, which required the use of the Microscan® Walkaway equipment, in addition to the molecular method (PCR). Results: the usual symptoms in pediatric and geriatric patients start with bloody diarrhea, nausea and vomiting; the localized study of this bacterium is carried out thanks to stool culture in specific or differential media, and for the molecular study techniques such as polymerase chain reaction (PCR) and electrophoresis are used. Conclusion: Despite the different methodologies of sowing in different media and different molecular techniques for the identification of this strain, the ideal medium is McConkey-Sorbitol Agar and for molecular identification the ideal technique is PCR.