Separación cromatográfica y caracterización espectroscópica de una mezcla de naringenina e isorhamnetina

Abstract

Naringenin and isorhamnetin are two closely related flavonoids found in citrus fruits, naringenin being predominant in oranges and isorhamnetin in lemons. Both compounds are characterized by their complex chemical structure with fused aromatic rings and a ketone group on the third ring. They are recognized for their powerful antioxidant and anti-inflammatory effect, as well as for their close relationship with the prevention of chronic diseases. The present study focuses on the application of chromatographic and spectroscopic methods for the separation and characterization of the flavonoid mixture composed of naringenin and isorhamnetin. The objective is to establish an analytical scheme, based on the analysis of updated scientific literature, that allows the chromatographic separation and spectroscopic characterization of a mixture of naringenin and isorhamnetin. Through a rigorous scientific approach and the consultation of updated literature, a comprehensive analytical scheme combining High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance Spectroscopy (NMR) has been designed. This analytical strategy will provide reliable and accurate results to identify and differentiate flavonoids in the mixture, allowing a qualitative and quantitative analysis of high scientific impact.