Detección de cryptosporidium en aguas crudas y tratadas de consumo humano mediante técnicas microbiológicas y moleculares

Abstract

The Cryptosporidium Protozoan affected humans are often those of the species and parvum hominis, where are the oocysts of these parasites resistant to the chlorine dosage that set out in the regulations of the Ministry of health in Colombia and the INEN standards in Ecuador, causing diseases in this way water how is cryptosporidiosis, disease prevailing in industrialized countries where ranges between 0.1 and 27.1%, (USA France, United Kingdom), which usually occurs in water parks and swimming pools public while in developing countries the results vary between 0.1 to 31.5% (Colombia, Chile, Brasil), due to the consumption of contaminated water, for which the importance of identifying Cryptosporidium in raw and treated waters using microbiological and molecular techniques such as control of water quality for human consumption. In the present study applied the colorimetric method and quantitative techniques Kinyou and TR-PCR for the detection of Cryptosporidium, in well water and drinking where well water results were obtained: 4/23 (17.39) and treated water: 5/38 (13.16%), These results give us important information because they reflect a difference between the waters of the well that is commonly consumed in rural areas and the waters of a treatment plant by comparing them it is concluded that raw waters are not suitable for human consumption.