Identificación de tiocolchicósido mediante cromatografía líquida de alta eficiencia

Abstract

Among the drugs in greatest demand for its therapeutic benefits in human development is folic acid, generally used in pregnancy to prevent neural tube defects in the fetus. There are medications that should not be administered during pregnancy, since they represent a high risk for the fetus. One of these drugs is thiocolchicoside, a muscle relaxant used to treat muscle spasms and trauma. The objective of the research is to identify thiocolchicoside in a batch of folic acid tablets through UV-Vis high-performance liquid chromatography. For this, a non-experimental descriptive methodology was used through the bibliographic review and analysis of scientific documents. Among the results of the investigation, it was determined that CLAE UV-Vis is the most appropriate technique for the identification of thiocolchicoside, since it allows the analysis of non-volatile and thermolabile substances with double bond chromophore groups in their structure such as thiocolchicoside, using the following analytical conditions, a mobile phase of high polarity such as water, methanol and acetonitrile at different concentrations, with a flow rate between 0.7 ml/min to 1.3 ml/min, and a C18 reverse phase column. Characteristics that allow the analyte to be separated and identified with high precision and sensitivity in retention times and maximum absorption value according to reference standards.