Identificación y cuantificación de ibuprofeno y diclofenaco sódico mediante cromatografía líquida de alta eficacia acoplada a un detector ultravioleta visible

Abstract

Ibuprofen and diclofenac sodium are medications used in the treatment of postoperative pain, feverish, inflammatory conditions and in some diseases such as arthritis and ankylosing spondylitis. In this research based on a bibliographic review we found that, to determine the purity of these drugs, it is recommended to apply high performance liquid chromatography by the reverse phase method, the mobile phase must contain polar substances such as acetonitrile or methanol while the stationary phase carries an apolar column such as octadecylsilane bonded to silica particles (C18). Taking advantage of the presence of chromophoric groups in the analytes, with the help of the UV-VIS detector it can be identified and, applying the external standard method, it is quantified. This study aimed to determine the presence of ibuprofen and diclofenac sodium by high performance liquid chromatography coupled to an ultraviolet detector for their identification and quantification.