Aplicación de pruebas serológicas y moleculares para el diagnóstico de chorioretinitis causada por toxoplasma gondii

Abstract

Bacteria and parasites are distributed throughout the earth, such as toxoplasma gondii, which infests animals and people until it reaches its definitive host, the cat. This study aims to argue the usefulness of serological and molecular techniques through the primary bibliographic compilation, for the evaluation of the clinical diagnosis of toxoplasmosis in patients with chorioretinitis, the study is descriptive, considering the method of the chemiluminescent immunoassay practical case comparing between the PCR and Elisa techniques for the determination of toxoplasma gondii in 67 patients attending the consultation related to ocular chorioretinitis at the “Ramon Pando Freire” Ophthalmic Hospital. Conclusion: Ocular toxoplasmosis is the inflammation of the choroid and the retina that compromises the patient's vision, serological techniques are tests that help determine the antibodies to a disease and molecular ones have a higher sensitivity because they identify the DNA of the parasite. It should be noted that the Elisa technique is more beneficial for the patient in detecting the disease because it takes less cost and time, however the PCR technique has 97% sensitivity and a high level of specificity for ocular chorioretinitis.