Investigación de técnicas microbiológicas y moleculares para la detección de hongos causantes de infección.

Abstract

300 million years ago the first kinds of fungi, but as they have been mutating, several species have emerged, some more pathogenic than others, these take advantage of immunocompromised people and those who do not have good hygiene, without medical care and especially in overcrowded conditions. Ecuador is located in a temperate and cold zone that facilitates the growth and adaptation of these microorganisms such as molds and yeasts, causing desquamation, itching or rashes, known as dermatophytosis. The present work is based on the investigation of the fungus "ASPERGILLUS fumigatus" which causes infections in humans, using microbiological and molecular techniques to identify this fungus that is causing morbidity and mortality in patients. The reliable techniques that allow us to identify a fungemia are: blood cultures, Gram staining, mass spectrometry, ELISA immunoassay, all these in a maximum time of 3 days, saving time and money, finally DNA replication, needs more than 3 days and an additional economic investment for the identification of the microorganism.