Procedimiento para determinar la concentración fito celular de un cultivo mediante la utilización del instrumento biológico cámara de neubauer.

Abstract

Live food in the development of aquaculture activities is of great importance, since it serves as a basis for the feeding of fish, mollusks and crustaceans, especially in the larval and postlarval stages. The control of parameters that directly affect phytoplankton and zooplankton cultures allows maintaining a good survival and development of these organisms, regulating at the same time the quality and concentration of some nutrients such as vitamins, acids and amino acids necessary for the culture. However, it is important to identify the type of microorganism being cultured, as well as their concentrations, which is why the Neubauer chamber is used. The Neubauer chamber or also known as hemacytometer is a glass instrument, a thick plate with which cell counts of some microorganisms, whether spores, parasites or plankton, can be performed. They have an interesting feature and it is that it is composed of three zones, the central one with which the count is performed and two other zones at the top and bottom. It is important to recognize the way in which the assembly of the chamber should be carried out, since any error can directly influence the cell count, whether it is an insufficient filling, not cleaning the chamber or even not making a good dilution of the sample, so that if any of them should occur, it is necessary to disassemble the chamber again, clean it and place a new sample.