Identificación de staphylococcus pseudintermedius y gen de resistencia meca por medio de pcr de 86 hisopados corneales obtenidos de perros sanos de la ciudad de Riobamba.

Abstract

"The therapeutic management of ocular pathologies is based on the timely use of antibiotics to prevent irreversible effects that harms the ocular globe. Currently, It has been identified to S. pseudintermedius as an opportunistic bacterium that prevails in the normal microbiome of healthy dogs´conjunctiva and cornea. As a result of which poses a threat to the effective treatment of eye infections due to the evolutionary capacity of its strains, which acquires genetic characteristics of antimicrobial resistance. The identification of the mecA resistance gene in S. pseudintermedius allows understanding the existence of methicillin-resistant populations in healthy dogs, and developing strategies for the responsible use of antibiotics. The objective of this research was to identify these bacteria, through the sequencing of the spsJ gene in S. pseudintermedius and the mecA resistance gene, using the Nested PCR technique. Samples were obtained from 86 corneal swabs in healthy dogs from Riobamba city. Moreover, the study focused on determining whether there is a relation between the lacrimal production and the skull shape with the presence of the bacterium. For the data analysis, frequencies and associations were described by using Chi-square test. Thirthy-three percent of the samples were determined to be S. pseudointermedius, and five percent of the total samples have the mecA resistance gene. It did not determine an associative effect due to lacrimal production, age, sex or reproductive status, although there was some evidence that exists a greater likelihood of finding these bacteria in brachycephalic animals. These data become significant for timely medical decision-making in ocular pathologies and contribute to prevent infections as well as to enrich the global and local database on bacterial resistance. This is one of the first study took place in Ecuador and the first one done in canine ocular microbiome."