Determinación molecular del virus de la diarrea viral bovina mediante rt-pcr en bovinos de la provincia de El Oro.

Abstract

This study focuses on the molecular identification of bovine viral diarrhea virus (BVDV) in cattle from the province of El Oro, Ecuador, using the RT-PCR technique. Bovine viral diarrhea is a highly contagious disease that significantly impacts livestock productivity and reproductive health, leading to considerable economic losses. The research aimed to standardize the RT-PCR molecular technique for diagnosing BVDV. Several farms in the province of El Oro were sampled, and the samples were analyzed to determine the presence of BVDV. The results revealed a prevalence of 29% in the province, identifying the presence of BVDV in animals that, although not showing evident symptoms, were carriers and contributed to the spread of the virus. This study underscores the importance of implementing precise molecular techniques like RT-PCR to improve the detection and control of BVDV, especially in PI animals that may go unnoticed with traditional methods. The standardization of this technique in Ecuador could be established as the "Gold Standard" for BVDV diagnosis.