Identificación de salmonella enterica serovar typhimurium en huevos de gallinas ponedoras mediante método molecular PCR

Abstract

Salmonella is a gram-negative, facultative anaerobic bacillus that belongs to the Enterobacteriaceae family and comprises two types of species: Salmonella enterica and Salmonella bongori. Salmonella enterica is classified in more than 2500 serotypes, one of them is Typhimurium considered as pathogenic microorganism causing gastrointestinal diseases causing salmonellosis, its clinical manifestations are: headaches, hyperthermia, nausea, emesis, colic, muscle and joint pain. For the identification of Salmonella bacteria, the molecular method was investigated by the PCR technique, based on the DNA of this pathogen, being an accurate and efficient analysis, with low monetary cost. The methodology is descriptive, based on a bibliographic search of reliable sources such as SciELO, PubMed, Google Scholar, and scientific journals, which allowed the analysis of Salmonella enterica serovar Typhimurium. The PCR technique is able to differentiate the serovars with their virulence not only has a genetic change, on the contrary, may have a greater amount in relation to genes that are associated with virulence. For this purpose, samples can be taken from the eggshell, egg white and egg yolk, where it consists of two antigens Somatic O (Lipopolysaccharides) Flagellar H (Flagellar proteins). It is recommended to the authorities to give importance to this type of diseases that are harmful and to investigate in the corresponding establishments about the healthiness that they have in the animal hatcheries, carrying out theoretical examinations on the Good Manufacturing Practices.