Efectos de métodos de desinfección ex vitro-in vitro en ápices meristemáticos de plátano clon dominico.

Abstract

The Dominican clone plantain (Musa x paradisiaca) is an essential monoculture for local food, with susceptibility limitations to pests and pathogens that affect its production in the medium and long term than the availability of quality plant material. The objective of the study was to determine the response of meristematic tips of plantain clone Dominico exposed to ex vitro-in vitro disinfection methods that allow improving the quality and their survival under in vitro conditions. A Completely Random Design (DCA) was used with a total of twelve treatments combining ex vitro and in vitro disinfection, and use of antibiotics (Chloramphenicol, Tebuconazole and Azoxystrobin) for a total of 60 sword-type axillary buds of 40–50 cm in diameter. height from which the meristematic apices were extracted, evaluating the response of apices, contamination (bacterial and fungal), phenolization and activity and inactivity. The best survival response of the meristematic apices was disinfection with fungicide and bactericide ex vitro-in vitro and fungicide and bactericide ex vitro-fungicide in vitro. Without antibiotics ex vitro-bactericidal in vitro due to its regeneration capacity by having apical dominance at 28 days, which shows a better quality of the apex. The quality in the coloration and the proliferation of the apical dominance without ex vitro-bactericidal disinfection in vitro proves to be the best disinfection process for the in vitro establishment phase.