Diagnóstico de la patogenicidad de entamoeba histolityca mediante la técnica de reacción en cadena de polimerasa (PCR)

Abstract

Entamoeba histolytica, a protozoan that resides in the human gastrointestinal tract, causes asymptomatic infections in approximately 90% of its hosts, playing an important role in the spread of the parasite. The molecular techniques used for the diagnosis of the parasite found in scientific articles are based on DNA detection such as polymerase chain reaction (PCR), real-time PCR, nested PCR, conventional PCR and loop-mediated isothermal amplification (LAMP). Objective: Describe the PCR technique for the molecular diagnosis of Entamoeba histolytica through bibliographic compilation. Method: The present work is a descriptive study, based on the analytical method, for the search and compilation of information related to the molecular techniques applied for the diagnosis of the pathogenicity of Entamoeba histolytica, by reviewing articles from journals indexed in different databases. of recognized bibliographic data that help to solve the question of the practical reagent. Conclusion: The diagnosis of the pathogenicity of Entamoeba histolytica is possible through molecular techniques of which the polymerase chain reaction (PCR) was found, which facilitates epidemiological studies of this microorganism, based on the detection of its DNA.