Resistencia bacteriana a antibióticos en problemas de otitis canina (Cannis Lupus Familiaris).

Abstract

The research was carried out in the Microbiology Laboratory of the Faculty of Agricultural Sciences of the Technical University of Machala; The objective of the research is to evaluate bacterial resistance to commonly used antibiotics against otitis problems in canine patients (Cannis lupus familiaris). The purpose of the study carried out below is to analyze the different cases of Bacterial Resistance in processes of ear problems in dogs within the city of Santa Rosa, before the most common antibiotics used for otitis treatments. The investigation was carried out with a population of 46 canines of different breeds, different age groups and of which were taken into account since they present alterations, clinical signs and symptoms in Canine Otitis (OC) problems. The experimental study has used the method of collecting samples with swabs directly in the affected area of the Auricular Pavilion (PA) and External Auditory Cavity (CAE), the collected samples have been taken to the laboratory to be sown in mannitol and cetrimide culture media. for specific bacteria such as Staphylococcus pseudintermedius and Pseudomona aeruginosa bacterium, after 24h00 has passed, which is the time in the incubator to obtain bacterial colonies, where 30 of the samples tested positive for Staphylococcus pseudintermedius with a percentage of 65.22% and there was no bacterial growth of Pseudomona aeruginosa with a percentage of 0%, Bacterial cultures were performed where the antibiograms were evaluated for each positive sample with the Kirby Bauer Disk Diffusion Method. The methodological approach that contributed to meeting the objectives of the study is of a qualitative nature, since the Ear Clinical Report form was first filled out (see Annex 1). Regarding the research design, it was the experimental scientific method since diagnostic tests were carried out to determine bacterial resistance in otitis problems in affected dogs and with clinical signs. The main results according to the evaluation of the antibiograms of the 15 antibiotics (see table 6), susceptibility results of 457 susceptibility results where 364 were sensitive to Staphylococcus pseudintermedius with a percentage of 79.65% sensitivity, 41 of them also had an intermediate resistance with a percentage of 8.97% of all the antibiotics under study, for which they have an intermediate resistance to Staphylococcus pseudintermedius and of 53 results obtained from all the antibiotics under study are resistant to this isolated bacterium with a percentage of 11.38% of (see Graph 9).