Utilización de pcr para la detección de enfermedades que afectan a la larva de camarón blanco (<i> litopenaeus vannamei </i>)

Abstract

Shrimp farming is an activity with high economic performance, for which it has managed to develop a lot in a very short period, the technologies that have been developed give way to this activity growing day by day. But instead there are some delimitations that slow down this evolution a bit, just as modernization allows us to have much more production capacity, the diseases that occur during the development of shrimp are multiple, and some with great devastation in crops. There are several infectious agents that to date have caused a great decline in the economy of farms. There are multiple technologies for the detection of these diseases, some more effective than others, since their effectiveness, sensitivity, specificity and efficiency could be questioned. Therefore, the option of using molecular tools is described as the most suitable solution to be able to detect in a timely manner and have a more specific diagnosis, with a high index of sensitivity and simplicity of interpretation. This paper explains how molecular tests and specific primers are developed and designed. Likewise, the procedures used by the aforementioned tools with the purpose of identifying and perceiving the presence of pathological agents in selected tissues of white shrimp larvae are explained: the amplification of selected genomic portions, which are through the use of oligonucleotides Specific primers for polymerase chain reaction (PCR) or real-time PCR (qPCR).