Efectos antioxidantes de moringa oleifera lam en vitroplantas de banano clon williams en sistemas de inmersión temporal rita®

Abstract

Banana production is El Oro's second economic activity where 40 year old plantations require renovating their traditional systems with young and vigorous plants. Micropropagation, as an alternative to obtain these banana plants, proposes RITA® temporary immersion systems. Where extracts of Moringa oleifera LAM (MOL) demonstrate the existence of organs with great potential for antioxidant activity. The content of soluble phenols and antioxidants in different organs in MOL, as well as banana vitroplants rooted in Rita® were evaluated. These systems were evaluated in different immersion cycles 3, 6 and 8 cycles, as well as conventional semi-solid systems, allowing to evaluate the elicitation of rooted vitroplants to reduce oxidative stress in these conditions. The phenolic content obtained from 6.35 and 6.53 mg GAE / g Dry weight of leaf and flower extract, as well as antioxidant in leaf extract with 24.36% reduction of DPPH in MOL organs. The RITA® demonstrated that 8 immersion cycles allowed 54.16% longer root length compared to 3, 6 cycles and semi-solid, as well as 2.75 grams of fresh mass production different from the systems and 0.18 grams dry mass that equals 3 cycles and semi-solid . The elicitation of aqueous MOL leaf extracts in Rita® at 8 MOL immersion cycles allowed the phenol content to be potentiated 4.38 mg GAE / g Dry Weight and antioxidants by 12.22% reduction in DPPH, reducing the oxidative stress of Williams vitroplants in rooting phase.