Detección molecular de Ehrlichia canis en perros sintomáticos y asintomáticos con factores de riesgo asociados en el cantón Huaquillas (Ecuador)

Abstract

The present study was designed and executed to determine the presence of Ehrlichia canis in dogs with and without symptoms from the Huaquillas canton, Ecuador, using molecular and epidemiological diagnostic techniques. To do this, we worked with two study groups: Group A, which consisted of 30 dogs with positive antibodies for E. canis detected by the specific immunochromatographic test, and Group B, which included 30 dogs with asymptomatic E. canis infection but with present risk factors, such as a recent history of tick bite or previous diagnosis of ehrlichiosis. Molecular detection, especially PCR, is mainly used in situations where the clinical diagnosis is uncertain or when treatment must be initiated rapidly, given the possibility of serious complications such as damage to internal organs, bleeding or neurological problems. Molecular detection tools are techniques that allow the identification of specific genetic material of pathogens. The main tools are: 1. PCR (Polymerase chain reaction): Amplifies specific DNA sequences, allowing the detection of pathogens from biological samples. It is widely used due to its high sensitivity. Molecular detection tools are techniques that allow the identification of specific genetic material from pathogens. This study focuses on molecular detection by means of DNA amplification, using specific primers or Primers, for end-point PCR tests where the variables of race, sex, parasitic load and age were analyzed to identify the presence of the pathogen in both groups and to identify if there is any reasoning between them and contagion. us. The main tools are: 1. PCR (Polymerase chain reaction): Amplifies specific DNA sequences, allowing the detection of pathogens from biological samples. It is widely used for its high sensitivity.