Caracterización química y capacidad antioxidante de la muestra de propóleos ecuatoriano ep12

Abstract

Propolis is a resinous product made by bees, mainly Apis mellifera, from exudates or parts of different plants that are close to the hive. In general, propolis is chemically composed of: organic acids, flavonoids, phenols, terpenoids, enzymes, minerals and vitamins; responsible for its protective, preservative and therapeutic properties. The objective of this experimental work was to identify the major chemical components and the antioxidant capacity of the Ecuadorian propolis sample EP12. For which, in the first instance, the extract was fractionated through a Sephadex LH-20 column using methanol as the mobile phase. The 11 fractions obtained were analyzed by TLC and by HPLC/MS and fraction 1/4 was selected to continue with the purification process. Fraction 1/4 (0.4 g) was subjected to normal phase column chromatography with Silica gel as stationary phase, while the mobile phases were prepared using hexane, chloroform and methanol as solvents, gradually increasing the polarity. The fractions obtained were combined according to the results observed in TLC and HPLC/MS into 9 fractions (3/1-3/9).