Detección de micosis mediante técnicas PCR y MALDI-TOF

Abstract

Fungi are eukaryotic bodies, these microorganisms require an external agent to obtain their own food, they are called heterotrophs. They are divided into different species, one of them the mycosis or commonly called fungal infection, they usually affect tissue, skin, bones, hair, nails and even organs. Mycosis is classified: superficial mycosis, subcutaneous mycosis and deep or systemic mycosis. These diseases have invaded around 1,200 million inhabitants. In order to reach fungal detection, we have set ourselves the objective of analyzing the PCR and MALDI-TOF techniques for the determination of mycosis by means of a bibliographic review. To meet the objective, techniques and methods from different bibliographic sources were used. The PCR technique (polymerase chain reaction) is responsible for amplifying a short sequence of the mother chain by making simultaneous copies of the DNA strand with the help of temperature, reproducing in a few hours. The MALDI-TOF technique (matrix-assisted laser desorption/ionization with time-of-flight mass detection) in order to determine we must first perform a blood culture; the method is performed by preparing the identification sheet to obtain the protein profile compared to the patterns that are already in the database. As a result, it was possible to identify its high specificity and sensitivity in both techniques. It can be concluded that both the PCR and MALDI-TOF techniques are accurate and reliable when making a diagnosis, but unlike PCR, MALDI-TOF is more complex in its process.